First, you need to know bothe the excitation and emission wavelengths of your chosen fluorphore. You can check both spectrums for many common fluorphores at the following spectrum viewers:
- BD Spectrum Viewer
- Life Technologies Spectrum Viewer
- eBioscience Spectrum Viewer
- BioLegend Sprectrum Viewer
Next, you need to know which lasers and detectors are available through the Cytometry Core Facility. Check the configurations for:
If you're still not sure, please contact us and we'll be happy to help you figure it out.