10mM HEPES/NaOH, pH 7.4
1.8 mM CaCl2
Propidium iodide 100µg/ml in Binding Buffer
Wash 2x106 cells with PBS.
Dilute FITC-Annexin V to 1 µg/ml in binding buffer and resuspend cells in 1 ml of this solution (prepared fresh each time).
Incubate 10 min. in the dark at room temperature.
Add 10 µl of PI solution for a final concentration of 1 µg/ml prior to analysis.