The Cytometry Core Facility, operated and administered as a partnership between Arizona Research Laboratories, Division of Biotechnology and the Arizona Cancer Center, offers instrumentation to analyze, quantify and sort cells and particles in a fluid suspension.
Flow cytometry is a powerful tool that can measure functional and structural characteristics of heterogeneous mixtures of cells and particles in suspension. The cell sorting function can physically separate these cells into different classes. The Cytometry Core Facility gives researchers the capability to analyze and sort cells by differences in physiology, metabolism, morphology and other characteristics. The ability to distinguish different cell types is limited only by the ability to attach specific fluorescent dyes to the cells.
Three instruments are available for use:
The FACSCanto II (BD Biosciences, San Jose, CA) is an analyzer equipped with 2 lasers, 6 fluorescence channels and 2 channels for Forward Scatter and Side Scatter. This instrument is an analysis instrument only.
The FACSAria III (BD Bioscienes, San Jose, CA) is a cell sorter equipped with 5 lasers: 488nm, 633nm, 561nm, 405nm and 355nm, and offers 17 fluorescence detectors. The sorting function of this instrument enables the researcher to physically separate the individual cells and particles from a mixed population. Viable cells can be recovered for further study or culture. The addition of a Baker BioProtect III biosafety cabinet, in which the Aria is housed, allows for sorting of certain live human tissue and BSL2 agents.
The LSRII (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from 16 fluorescence parameters and has five lasers: 355nm, 405nm, 488nm, 532 nm and 640nm. This instrument is an anlysis instrument only.
The FACScan (BD Biosciences, San Jose, CA) After a long and productive career, the FACSan is now retired.
We also offer information about new techniques, instrumentation and applications of flow cytometry provided through Biotechnology-sponsored workshops and seminars.
Individual consultation services are available to discuss applications and methodologies specific to your project.